Figure 5.

Western immunoblot analysis of NADPH oxidase components. Purified mature bone marrow neutrophils were sonicated into loading buffer, and protein samples were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (7.5 to 15%). Immunoblots were probed with antibodies directed against NADPH phox components. Blots were reprobed with GAPDH as a loading control, and relative levels of proteins was determined by using densitometric scanning. Data show the Western immunoblot results from four WT and four LFKO mice. The graph shows the quantitation of the relative amounts of the proteins (±SEM) in WT and LFKO samples after normalization against GAPDH. Note: For the p40^phox Western, two gels were run with the first gel containing duplicate samples from two WT and two LFKO mice and the second gel containing duplicate samples from another two WT and two LFKO mice. The transferred blots were split in half and one half probed with p40^phox and the other half probed with GAPDH because these two proteins are similar in size.