Figure 7.
Primary granule myeloperoxidase secretion and CD11b up-regulation is normal in LFKO neutrophils. A: Purified bone marrow neutrophils (5 × 106/ml) from WT and LFKO mice were incubated with 5 μg/ml cytochalasin B and 5 μmol/L fMLP for 15 minutes, and supernatants were collected and analyzed for secretion of (MPO). Data represent the mean percentage of MPO released (±SEM) relative to total cellular MPO. Neutrophils from six WT and six LFKO mice were used for this analysis. B: Expression of CD11b on the surface of resting and PMA-activated neutrophils was detected by staining with phycoerythrin-conjugated anti-CD11b followed by flow cytometric analysis. Data represent the mean (±SEM) of the MFI for CD11b on WT and LFKO neutrophils (n = 6 per genotype).