Figure 1.

CCN6 expression is associated with E-cadherin protein in human invasive carcinomas of the breast. TMA sections stained with CCN6 and E-cadherin antibodies showing a representative invasive ductal carcinoma with high cytoplasmic CCN6 and positive E-cadherin at the cytoplasmic membranes (top) and another invasive ductal carcinoma with low CCN6 and reduced/neg E-cadherin (bottom). Immunohistochemistry was analyzed using pathology-based interpretation and an automated image analysis system (ChromaVision Medical Systems), consisting of an automated robotic bright-field microscope linked to a computer through a Microsoft Windows NT-based software interface. This system allows more reproducible and objective scoring than manual interpretation and provides a continuous rather than categorical score. Magnification, ×400.