Figure 6.

UVB inhibits the development of CD8⁺ T cells in the ear skin of resting mice 10 weeks after sensitization. Ox-sensitized unirradiated and UVB-irradiated mice were challenged with either Ox or TNCB before being rested. T cells were examined in the ear skin of resting mice at 10 weeks after sensitization. A: Representative dot plots of skin-derived cells gated on CD45⁺ expression. A prominent CD8⁺ T-cell population is present in the ear skin of unirradiated sensitized mice, but is absent in UVB-irradiated sensitized or unsensitized mice. Percentages of gated CD45⁺ cells are shown. B: The number of CD8⁺ T cells recovered from the ear skin of sensitized mice. No CD8⁺ T cells were detected in Ox-sensitized but TNCB-challenged mice. n = 9 mice pooled from three individual experiments, means + SEM are shown. *P < 0.05 comparing no UVB with UVB mice.