Figure 8.

CD8⁺ T cells in ear skin maintain a GFP⁻ phenotype 5 weeks after sensitization. Unirradiated and UVB-irradiated T-GFP mice were sensitized with Ox and 7 days later, ears were challenged with Ox. Mice were rested for 5 weeks after sensitization before T cells were examined in the ear skin. A: Representative dot plots of skin-derived cells gated on CD45⁺ showing CD3⁺CD8⁺ T cells isolated from T-GFP ear skin in unirradiated and UVB-irradiated sensitized mice. Percentages of gated CD45⁺ cells are shown. A skin sample spiked with lymphoid cells is presented as a positive gating control. B: The GFP expression of gated CD3⁺CD8⁺ skin-derived T cells is predominately GFP⁻ compared to lymphoid-derived GFP⁺ T cells. C: The number of GFP⁻ and GFP⁺ CD8⁺ T cells in unirradiated and UVB-irradiated mice. n = 5 to 9 mice pooled from two experiments, means + SEM are shown. *P < 0.05 comparing unirradiated with UVB mice.