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Effects of zinc deprivation on cell proliferation-related proteins in HepG2 cells. TPEN was added at 2 μmol/L with or without zinc supplementation at 50 and 100 μmol/L elemental zinc. Cells were incubated for 4 days. A: Western blotting. B: Densitometry analysis of Western blotting bands. Results are means ± SD (n = 2–3). Significant differences (P < 0.05) among a, b, and c are determined by ANOVA.
