Figure 7.

Co-localization of GM-Mac with lipid. Co-localization of fluorescence oil-red-O staining of lipid (A and D) and fluorescence immunostaining of CD68 (red fluorescence, B and E) and CD14 (green fluorescence, C and F) in an advanced atherosclerotic lesion is shown. Cells were visualized by DAPI nuclear staining (blue fluorescence). The boxed region is shown at higher magnification at the bottom and demonstrates lipid-containing CD68⁺/CD14⁻ macrophages (ie, GM-CSF phenotype). Scale bars: 20 μm (C; also applies to A and B); 5 μm (F; also applies to D and E).