Table 3.
Quantitative PCR for Selected Lipid-Related Genes in M-Mac and GM-Mac
| Gene | Fold difference
|
|
|---|---|---|
| qPCR | Microarray | |
| ABCG1 | 18.7 | 5.6 |
| CCR7 | 17.3 | 4.0 |
| PPAR-γ | 7.4 | 9.8 |
| LXR-α | 5.1 | 5.4 |
| ApoE | 3.0 | 5.1 |
| ABCA1 | 1.4 | −1.7 |
| PPAR-α | 1.1 | 2.0 |
| ACAT1 | −1.2 | −1.1 |
| LXR-β | −1.4 | −2.0 |
The genes listed above were investigated with quantitative PCR. A positive fold difference indicates an up-regulation in GM-Mac relative to M-Mac while a negative fold difference indicates a down-regulation. The quantitative PCR measurements correlated with the gene expression microarray (R2 = 0.57). The results are the means of three monocyte donors.