Figure 9.

Increased PtdIns(4,5)P₂ Levels of Seedlings Overexpressing PIP5K3.

Lipids were extracted from uninduced (−) and induced (+) ER8-GFP and ER8-PIP5K3 seedlings, analyzed by thin layer chromatography, and quantified by phosphoimaging. Each sample is represented by three seedlings, with analysis performed in triplicate.

(A) Thin layer chromatography image of ³²P-labeled phospholipids. Results from a representative experiment are shown (n = 3). The positions of phospholipids and the origin are marked. PtdCho, phosphatidylcholine; PtdEtn, phosphatidylethanolamine; PtdGro, phosphatidylglycerol.

(B) Longer exposure thin layer chromatography image of ³²P-labeled PtdIns(4,5)P₂.

(C) Quantification of ³²P-labeled PtdIns(4,5)P₂. The means and sd are indicated by the white and gray bars and error bars (n = 3), respectively.