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In Vitro Enzyme Assays of Wild-Type GA3ox3 and GA3ox4 Proteins.
Gas chromatography–selected ion monitoring showing the conversion of GA9 to GA4 by MBP-GA3ox3 fusion protein (middle panel) and MBP-GA3ox4 fusion protein (bottom panel). Cell lysates containing MBP were used as a negative control (top panel). GA9 and GA4 were identified as GA9-methyl ester (GA9-Me) and GA4-methyl ester-trimethylsilyl ether (GA4-MeTMS), respectively, after derivatization. m/z, mass-to-charge ratio.
