Figure 5.

Cellular Location of GA3ox-GUS Expression during Flower Development.

(A) to (C) Transverse sections of anthers expressing GA3ox3-GUS, GA3ox4-GUS, and GA3ox2-GUS, respectively. Numbers at the bottom left corner of each picture indicate the corresponding anther developmental stages according to Sanders et al. (1999). Arrowhead, tapetum; arrow, pollen grains.

(D) Longitudinal section of inflorescence apex of GA3ox1-GUS. m, inflorescence meristem. 2, stage 2 flower primordia; 3, stage 3 flower; 7, stage 7 flower. Flower stages were determined according to Bowman (1994).

All sections were viewed under dark-field microscopy, and the X-Gluc staining is pink. Bars = 50 μm.