Figure 6.
Changes of phyB Levels in pif3 Mutant Seedlings Precede the Changes in Hypocotyl Elongation.
(A) Time course of hypocotyl length and phyB level in wild-type Col-0 and pif3-3 mutant seedlings. Seedlings were germinated and grown as described in Methods (Monte et al., 2004). Germination was induced for 3 h in white light followed by 21 h of darkness, and the seedlings were then moved to Rc (7.7 μmol·m−2·s−1) for up to 36, 48, 60, or 96 h after inducing germination before measuring hypocotyl length (top) and phyB levels (bottom). Mean values of hypocotyl length are representative of at least 25 seedlings. phyB normalized to tubulin (phyB/tub) was quantified as in Figure 5B, and mean values from three biological replicates are shown (bottom). Bars represent se. P values from a t test analysis for differences between wild-type and pif3-3 mutant seedlings are shown.
(B) Immunoblots corresponding to all of the replicates (rep.) analyzed in (A), bottom panel, are shown.