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pH Dependence of the Enzymatic Activity of TPS23.
The catalytic activity of the purified enzyme was measured in the presence of 10 mM Mg2+. The pH values were adjusted with the following buffers: pH 5.0 and 5.5, acetate buffer (100 mM); pH 6.0, MES buffer (100 mM); pH 6.5 to 9.5, bis-Tris-propane buffer (100 mM); pH 10.0 to 11, CAPS buffer (10 mM). Means ± se of triplicate assays are shown.
