Effects of HDL oxidation to various stages by OCl− on protein cross-linking analyzed by SDS PAGE. HDL (1 mg/mL protein in buffer B) were incubated with various concentrations of NaClO (see Methods); lane numbers correspond to approximate oxidant:HDL molar ratios of (1) 7.5:1, (2) 25:1, (3) 50:1, (4) 100:1, (5) 200:1, (6) 400:1; 0 stands for nHDL. The gel was stained with Coomassie blue.