The effect of HU on germination and appressoria formation in five different Colletotrichum species. Fungi were germinated on the surface of a Petri dish in water (C. magna, C. coccodes, C. acutatum) or in PDB medium (C. gloeosporioides, C. musae). Spore concentration was 105/ml in all species. Percentage germination and appressoria formation were determined after 7 h (C. gloeosporioides, C. coccodes), 9 h (C. acutatum, C. musae), or 13 h (C. magna). In each sample 150 spores were counted, with two independent samples per experiment. Appresoria percentage is the percentage of germ tubes that formed appressoria.