Figure 1.

Diagram of reporter constructs. Diagram of reporter construct integrated into the non-transcribed spacer of the ribosomal DNA locus of Trypanosoma brucei. The GFP and mRFP genes were exchanged as a HindIII-BamHI cassette. The boxes represent the genes indicated and the grey boxes represent the rRNA intergenic region, part of which was used for targeting the construct to this location. Thin line represents plasmid construct. The arrowheads show positions of primers used to check integration site. H = HindIII, B = BamHI.