Table 1.

P24 production in HIV-1 persistently-infected cell lines exposed to TNF-α or PMA

Cell line and treatment	0 h	24 h	48 h
J1.1 Cells	14.07 ± 0.01	14.14 ± 0.05	18.26 ± 0.80
J1.1 Cells + TNF α	14.08 ± 0.01	12.86 ± 0.90	152.04 ± 1.50
U1 Cells	1.06 ± 0.03	1.60 ± 0.03	4.18 ± 1.11
U1 Cells + PMA	1.05 ± 0.03	51.96 ± 9.20	191.76 ± 0.48

HIV-1 persistently-infected lymphoid J1.1 and monocytic U1 cells were treated with TNF-α and PMA respectively in order to stimulate viral replication. P24 antigen was determined at 0, 24 and 48 h in cell supernatants by ELISA (HIVAG-1 Monoclonal, Abbot Laboratories). Values correspond to p24 antigen per 200,000 cells, expressed in J1.1 as pg/ml and in U1 Cells as ng/ml.