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. Author manuscript; available in PMC: 2008 Mar 29.
Published in final edited form as: J Biol Chem. 1999 Oct 1;274(40):28584–28589. doi: 10.1074/jbc.274.40.28584

Fig. 2. Northern blot analysis of MT-I expression in response to heavy metals in Ku-80 cells after treatment with 5-AzaC.

Fig. 2

A, Ku-80 (R80-1) cells were exposed to increasing concentrations of 5-AzaC for 72 h followed by CdSO4 (30 μm) treatment, total RNA was isolated and subjected to Northern blot analysis with α-32P-labeled, random-primed, mouse MT-I cDNA as probe. B, Ku-80/R80-1 and R80-6 cells were exposed to 20 μm 5-AzaC for 72 h, followed by treatment with 30 μm CdSO4. Total RNA was isolated and subjected to Northern blot analysis as above. C, Rat-1 and Ku-80 cells were treated with 30 μm CdSO4 following treatment with 20 μm 5-AzaC. RNA was isolated and subjected to Northern blot analysis as above. 18 S ribosomal RNA was used in all samples to confirm equal loading of RNA.