EGCG decreases nuclear, cytosolic, and membrane-associated β-catenin protein expression, as well as membrane-associated E-cadherin. (A) Representative immunoblot data of cytoplasmic and nuclear fractions of HEK293 cells transfected with S33Y-β-catenin and probed with anti-β-catenin and anti-β-actin antibodies. (B) Western blot of membrane-associated proteins from HEK293 cells transiently transfected with S33Y-β-catenin and probed with anti-β-catenin, anti-E-cadherin and anti-β-actin antibodies. (C) Densitometry data for results shown in (A) and (B), normalized to β-actin, showing relative expression levels of β-catenin and E-cadherin with increasing concentration of EGCG.