Fig. 5.

SFN increases multi-caspase activity and causes cell cycle arrest in prostate epithelial cells. BPH-1 cells (A) and PC-3 cells (B) were harvested 48 h after treatment with 0 or 15 μM SFN, as indicated, and the attached cells were examined for multi-caspase activity using a Guava PCA. Results represent mean ± SD, n = 3, from experiments conducted on two or more separate occasions. BPH-1 (C) and PC-3 (D) cells were treated as above, and cell lysates were immunoblotted for procaspase-3. In (E) BPH-1 and PC-3 were harvested, fixed and stained for cell cycle analysis. Attached and floating cells were fixed in 70% ethanol and stained with propidium iodide, and cell cycle kinetics was examined using the Guava PCA, followed by data analysis with Multi-Cycle software. Results indicate mean ± SD, n = 3; **P <0.01, ***P <0.001.