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. Author manuscript; available in PMC: 2008 Mar 29.
Published in final edited form as: FASEB J. 2003 Oct 2;17(15):2278–2280. doi: 10.1096/fj.03-0040fje

Figure 1.

Figure 1

VEGF stimulates the expression of ETS1 without changes in its phosphorylation. (A) Northern blot analysis of total RNA from HUVEC treated with or without VEGF for the times indicated and probed for ETS1 and actin. (B) Northern blot analysis of HUVEC treated with or without actinomycin D (10ng/ml) and stimulated with VEGF (20ng/ml) for 3 hours. (C) Nuclear run-on analysis of nuclei isolated from HUVEC treated (+) or untreated (−) with VEGF for 3 hours and pulse labeled with γ–32PUTP. Isolated nascent RNA was used to probe dot blots containing either vector alone or ETS1 cDNA (1cg/dot). (D) Western blot analysis of total protein isolated from HUVEC treated with VEGF for the times indicated, immunoprecipitated with anti-ETS1 antibody and immunoblotted with antibodies against either ETS1 or phospho-threonine. An immunoblot for the p85 subunit of PI3K serves as loading control.