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. Author manuscript; available in PMC: 2008 Mar 29.
Published in final edited form as: FASEB J. 2003 Oct 2;17(15):2278–2280. doi: 10.1096/fj.03-0040fje

Figure 2.

Figure 2

Pharmacological inhibition of VEGF-induced ETS1 expression. (A) Western blot analysis of total protein isolated from serum and growth factor starved HUVEC that are pretreated with DMSO (C), PD098059 (PD) or wortmannin (WTM), then with (+) or without (−) VEGF for 10min. Duplicate blots were probed with antibodies against phosphorylated Akt (Akt-P) and Erk1 and 2 (p42/p44-P). Blots were reprobed with antibodies against total Akt and Erk 1 and 2. (B) Northern blot analysis of total RNA isolated from serum and growth factor starved HUVEC that are pretreated with PD098059 (PD) or wortmannin (WTM) then with (+) or without (−) VEGF for 3 hours and probed for ETS1 and reprobed with actin. Numbers are normalized relative densitometric units.