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. 1998 Jun 23;95(13):7813–7818. doi: 10.1073/pnas.95.13.7813

Figure 9.

Figure 9

Protein blots showing GFP compartmentalization in tobacco. The mitochondrial and cytosolic fractions (A) and the chloroplastic fractions (B) of control, untransformed plants (lanes C), tobacco transformed with pBI-TP-GFP5 (lanes +TP) or pBI-GFP5 (lanes −TP) were probed with GFP antibodies. Ten micrograms of protein from crude mitochondrial (Intact Mit) or chloroplastic (Intact Chl) fractions; 25 μg of soluble proteins from purified mitochondria (Mit Matrix) or chloroplasts (Chl Stroma); 20 μg of insoluble membrane fraction of purified mitochondria (Mit Memb) or chloroplasts (Chl Memb); and 100 μg of soluble proteins from the cytosolic fraction (Cytosol) were loaded per lane. (C) Immunodetection of the mitochondrial IDH in untransformed tobacco. Lane 1, cytosol (100 μg); 2, mitochondria (25 μg); and 3, chloroplasts (25 μg).