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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2007 Oct 10;44(3):367–374. doi: 10.1016/j.freeradbiomed.2007.09.021

Figure 4. Bcl-XL blocks Bax-dependent hyperoxic cell death.

Figure 4

(A) Expression of Bax and Bcl-XL in HCT116 wt and Bax− cells mock transfected or treated with siRNAs targeting luciferase (Luc) or Bcl-XL prior to hyperoxic exposure for 4 days with actin used as a loading control. Immunoblots were representative of three separate experiments. (B) Percentage of cell death in HCT116 wt and Bax− cells transfected with Bcl-XL siRNAs then exposed to hyperoxia for 4 days. (C) Representative DNA histograms HCT116 wt cells transfected with Bcl-XL siRNAs then exposed to room air or hyperoxia for 4 days. (D) Mean average of HCT116 wt and Bax− cells in G1, S and G2/M after Bcl-XL siRNA treatment cultured in room air or hyperoxia for 4 days (*p=0.02 compared between cell lines; †p=0.0004 compared between siRNA treatments; n=4)‥