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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Protein Expr Purif. 2007 Oct 1;57(2):271–279. doi: 10.1016/j.pep.2007.09.014

Fig. 2.

Fig. 2

Purification of AID from E. coli lysate. (A) SDS-PAGE analysis with Coomassie staining. Lane 1 shows the total soluble lysate. Lane 2 shows the proteins from the soluble lysate that were not retained after passage over the GST Sepharose High Trap HP affinity column. Lane 3 shows the proteins that were retained and subsequently eluted from the GSTrap HP affinity column. Lane 4 shows the protein products obtained after digestion with GST-PreScission™ protease. Lane 5 shows purified AID resolved by Superdex™ 75 size-exclusion chromatography. (B) Superdex™ 75 size-exclusion purification of AID after digestion with GST-PreScission™ protease.