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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Protein Expr Purif. 2007 Oct 1;57(2):271–279. doi: 10.1016/j.pep.2007.09.014

Fig. 4.

Fig. 4

AID inhibition of PDK1-catalyzed T229 phosphorylation of λPP-treated His6-S6K1αII(ΔAID)-T389E. Western analysis was used to follow the time dependence (0, 5, 15, and 30 min) for T229 phosphorylation that occurs on incubation of 1 μM λPP-treated His6-S6K1αII(ΔAID)-T389E substrate with 20 nM PDK1 enzyme. Time dependences are shown for the (A) phosphorylation reaction alone; (B) the phosphorylation reaction in the presence of 5 μM AID; and (C) the phosphorylation reaction in the presence of either 5 μM or 20 μM AID(D2ED).