Table 1.

Purification of the C-terminal AID construct of S6K1 from E. coli^a

Purification (Step)	Volume (mL)	Concentration (mg/mL)	Yield (mg)	Purification (fold)
Crude lysate	25	15 ± 2b	375 ± 50	N/A
GST affinity	10	1.7 ± 0.2b	17 ± 2	22
Superdex™ 75	5	0.40 ± 0.05c	2.0 ± 0.2	150

^aAll values are reported for purification from expression in 1 L of E. coli.

^bProtein concentrations were measured by Bio-Rad protein assay.

^cPurified AID concentrations were measured using ε_{280 nm} of 9900 M^-1 cm^-1 and converted to mg/mL using the calculated molecular mass of 11,988 Da.