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. Author manuscript; available in PMC: 2009 Feb 7.
Published in final edited form as: Cell Stem Cell. 2008 Feb 7;2(2):151–159. doi: 10.1016/j.stem.2008.01.004

Figure 1. Derivation of iPS cells using an inducible lentiviral system.

Figure 1

Somatic cells harboring a GFP reporter driven by the endogenous Oct4 or Nanog promoters were infected with tet-inducible lentiviral vectors carrying the cDNAs of Oct4, Sox2, Klf4 and c-Myc (A). The addition of doxycycline after infection induced lentiviral expression and subsequently led to reprogrammed, GFP positive iPS cell colonies (B). The iPS cells were pluripotent and contributed to viable chimeras after injection into BALB/C host blastocysts as indicated by coat color (C, D). White mice are non-chimeric BALB/C animals, whereas mixed coat color mice are chimeric.