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. Author manuscript; available in PMC: 2009 Feb 7.
Published in final edited form as: Cell Stem Cell. 2008 Feb 7;2(2):151–159. doi: 10.1016/j.stem.2008.01.004

Figure 3. SSEA1 expression marks an intermediate state of reprogramming.

Figure 3

SSEA1+/GFP− cells were sorted with an APC conjugated SSEA1-antibody after 21 days of transgene expression and seeded on feeder MEFs (A). GFP positive ES-like cell colonies were observed ten days after sorting (B). Supplementation of the media with dox for an additional three days after sorting increased the percentage of SSEA1+/GFP−cells that gave rise to GFP positive iPS cell colonies (C). Numbers from two independent experiments for Oct4-GFP and Nanog-GFP were averaged. Error bars indicate standard deviations. Confirmation of GFP-positive cells in the sorted SSEA1+/GFP− populations eleven days after initial sorting and seeding on feeder MEFs (D).