Fig. 7.

The Mitf^mi-rw allele partially rescues the Chx10^orJ mutant phenotype. Eyes from newborns of the indicated genotypes were sectioned and processed for in situ hybridization with a pan-Mitf probe (A–D), CYCLIND1 immunofluorescence (E–H), or PAX6/TUJ1 double immunofluorescence (I–L). Compared with wild type, Mitf^mi-rw/mi-rw retinas appear normal both in thickness and in staining. In contrast, Chx10^orJ/orJ retinas retain Mitf expression and are severly hypoplastic, with a pigmented monolayer replacing the retina particularly in the distal part (B). Moreover, they show fewer CYCLIND1-positive and PAX6-positive cells (F,J). Eyes from Mitf^mi-rw/mi-rw;Chx10^orJ/orJ double mutants, however, have retinas of relatively normal appearance and thickness even though their PAX6 staining and lamination is still abnormal (C,G,K). Brackets at the bottom mark the thickness of the retina.