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Nrg 1-β-1 induces the formation of nuclear bag fibers in vitro. (A-C) Representative images used to tabulate the number of bag fibers present in treated and untreated cultures and total myotubes (red arrows indicate counted bag fibers), (D) continuous double bag fibers were observed sporadically in treated cultures (red arrows) and represent a physiologically relevant phenomenon. Scale bars = 100μm.
