Table 1.
Serum-free medium composition in Neurobasal used for myocyte growth and intrafusal fiber differentiation.
| Component | Concentration | Company | Catalog Number |
|---|---|---|---|
| B27 | 20μl/ml | Gibco | 17504-044 |
| Glutamax | 10μl/ml | Invitrogen | 35050-061 |
| Antibiotic/Antimycotic | 10μl/ml | Invitrogen | 15240-062 |
| aFGF | 20ng/ml | Invitrogen | 13241-013 |
| VEGF 165 | 20ng/ml | Invitrogen | P2654 |
| G5 (10×) | 2μl/ml | Invitrogen | 17503-012 |
| h BDNF | 20ng/ml | Cell Sciences | CRB 600B |
| h GDNF | 20ng/ml | Cell Sciences | CRG 400B |
| r CNTF | 50ng/ml | Cell Sciences | CRC 401B |
| h CT-1 | 20ng/ml | Cell Sciences | CRC 700B |
| NT-3 | 20ng/ml | Cell Sciences | CRN 500B |
| NT-4 | 20ng/ml | Cell Sciences | CRN 501B |
| De-N-sulphated | |||
| N-acetylated heparan sulfate | 80ng/ml | Sigma | D9809 |
| Vitronectin | 100ng/ml | Sigma | V0132 |
| h β-NGF | 10ng/ml | R&D Systems | 256-GF |
| h Nrg 1-β-1* | 100ng/ml | R&D Systems | 396-HB |
*
Supplemental component added only as indicated.