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. Author manuscript; available in PMC: 2008 May 30.
Published in final edited form as: Mol Cell. 2007 Nov 30;28(4):665–676. doi: 10.1016/j.molcel.2007.11.010

Figure 3.

Figure 3

CHD1 is stably associated with the SF3a sub-complex of U2 snRNP. A) Purification scheme of CHD1/SF3a complexes. B) Immunoaffinity purification using the indicated antibodies for precipitation (top) and detection by western blotting (side). The input material was derived from the DEAE-5PW elution indicated as immunoaffinity in (A). C) Analyses of the gel filtration fractions from the Superose 6 PC 3.2/30 labeled in (A). Western blotting was performed using the indicated antibodies (side). Sizes are indicated (kD). D) Co-immunoprecipitation experiment using IgG or anti-CHD1 antibodies together with recombinant CHD1 and 35S-translated SF3A1, SF3A2, or SF3A3 protein. A Coomassie stain of recombinant CHD1 is indicated in the left panel.