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. Author manuscript; available in PMC: 2008 May 30.
Published in final edited form as: Mol Cell. 2007 Nov 30;28(4):665–676. doi: 10.1016/j.molcel.2007.11.010

Figure 5.

Figure 5

CHD1 and H3K4me3 modulate the association of SF3a on chromatin. A) Western blotting of whole cell extracts from control, CHD1, or ASH2 siRNA-treated cells (indicated on top). B) Sub-cellular fractionation from cells treated with the indicated siRNA (top). Western blotting was performed using the labeled antibodies (side). MEK2 is a cytoplasmic protein used as a control for the integrity of the fractionation. C) Chromatin immunoprecipitation on the cyclin D1 gene. A scheme of the cyclin D1 gene is indicated on top. The antibodies used for immunoprecipitation are labeled. D) Chromatin immunoprecipitations on the cyclin D1 gene derived from cells treated with control or CHD1 siRNAs. The antibodies used for immunoprecipitation are labeled (side). C) Graphical representation of three independent experiments as performed in (D). Results from three independent experiments are shown represented by the mean and standard error.