Figure 3. The effect of storage and vortexing.

Samples were pooled from 3 individuals. Amount of urinary exosome-associated protein (200,000 × g pellet) (A), Coomassie blue-stained gel of equal fraction volume of protein (B), Western blot (10 :l aliquot) for NHE3, TSG101, ALIX and AQP2 (C).