Phosphorylation of FOXO3a decreases in different HIV-1 strains infection of human MDM. A, Human MDM were infected by HIV-1ADA, HIV-1JRFL, HIV-1BAL strains. Cell lysates were collected 5 days after infection and subjected to Western blotting for p-FOXO3a (phosphorylated FOXO3a) and FOXO3a (total FOXO3a). Control denotes uninfected cells. β-actin was used as a loading control. B, Viral strain infections were treated with AZT (5 μM). C and D, Results of A and B were densimetrically quantified as a ratio of p-FOXO3a to FOXO3a and presented as percentage of control in C and D, respectively. Average ± SD of three independent experiments from three donors were shown. **, p < 0.01 compared with control.