Skip to main content
. Author manuscript; available in PMC: 2009 Jan 15.
Published in final edited form as: J Immunol. 2008 Jan 15;180(2):898–906. doi: 10.4049/jimmunol.180.2.898

FIGURE 6.

FIGURE 6

DN FOXO3a or FOXO3a siRNA reduces apoptosis in HIV-1-infected MDM. A, Apoptosis was detected by DNA fragmentation apoptosis ELISA after 48 h of DN FOXO3a delivery to HIV-1-infected MDM. B, Cell viability was detected by MTT assay after 3 day of DN FOXO3a delivery to HIV-1-infected MDM. Results shown in A and B are the average ± SEM of triplicates performed with three different donors. **, p < 0.01 compared with Ad-GFP control; ##, p < 0.01 HIV-1/Ad-DN-FOXO3a vs HIV-1/Ad-GFP. C, FOXO3a knockdown was determined by Western blotting. β-actin was used as a loading control. D, Densimetrically quantified data of C are shown. **, p < 0.01 compared with nonspecific siRNA control; ##, p < 0.01 compared with nonspecific siRNA control in HIV-1-infected MDM. E, Apoptosis was detected by DNA fragmentation apoptosis ELISA in MDM after 48 h of siRNA transfection. F, Cell viability was detected by MTT assay after 72 h of siRNA transfection. Results shown in E and F are the average ± SEM of experiments performed in triplicate (n = 3 donors). **, p < 0.01 compared with nonspecific siRNA control; ##, p < 0.01 compared with nonspecific siRNA control in HIV-1-infected MDM.