Skip to main content
. Author manuscript; available in PMC: 2009 Jan 15.
Published in final edited form as: J Immunol. 2008 Jan 15;180(2):898–906. doi: 10.4049/jimmunol.180.2.898

FIGURE 7.

FIGURE 7

Reduced phosphorylation of FOXO3a in HIV-1-infected macrophages is mediated through inhibition of Akt-1. A, Human MDM were treated with 0.5 or 5 μM of PI3K inhibitor LY294002, and cell lysates were collected 2 h later. DMSO was used as a vehicle control without LY294002 treatment. Phosphorylated FOXO3a, total FOXO3a, and β-actin were determined by Western blotting. B, After 2 days of infection by HIV-1, MDM were infected with adenovirus expressing constitutively active Akt-1 (ad-myr-Akt) for 72 h at 10,000 MOI, p-FOXO3a, total FOXO3a, Akt-1, and β-actin were determined by Western blotting. C, Ratio of p-FOXO3a to FOXO3a was generated from the densimetrical quantification of B with four different donors. p < 0.05, compared with GFP vector control; #, p < 0.05, HIV-1/Ad-myr-Akt vs HIV-1/Ad-GFP.