Fig. 2.
The detection limit of the suspension array with 15 bead sets for Listeria monocytogenes NICPBP54001. PCR product of 9.3×107 CFU/ml at 26 cycles and amplicons at 35 thermal cycles from different concentrations (9.3×107, 9.3×106 and 9.3×105 CFU/ml) and template-negative control (PCR NC), were hybridized to the multiplex array and analyzed by LiquiChip reader. On the basis of 9c positive signals, Listeria monocytogenes was identified. The detection limit at 35 cycles for Listeria monocytogenes was 9.3×106 CFU/ml
C 1: 9.3×107 (26 cycles); C 2: 9.3×107 (35 cycles); C 3: 9.3×106 (35 cycles); C 4: 9.3×105 (35 cycles); C 5: PCR NC (35 cycles)