Table 1. Cell Samples in ML Experiments.
Mouse | Cell Type | Source | Expansion | % Mut. | % Amp. |
ML2 (26) | Satellite cell (10) | EDL (2): Left (2 fibers) | Culture | 9.8% | 85.4% |
Age: 10w | SOL (2): Left (1, 1 fiber) | ||||
Right (1, 1 fiber) | |||||
MAS (6): Left (4 fibers) | |||||
Kidney SC (8) | Kidney | Culture | 13.4% | 38.7% | |
B-cell (8) | Spleen | WGA (G-Plex) | 18.2% | 24.6% | |
ML4 (25) | Satellite cell (12) | GAS (8): Left (2 fibers) | Culture | 13.3% | 69.7% |
Age: 13w | EDL (2): Right (1 fiber) | ||||
MAS (2): Left (1 fiber) | |||||
Mesenchymal SC (5) | Right Tibia (3) | Culture | 8.5% | 72.9% | |
Right Femur (2) | |||||
Hematopoietic SC (2) | Left Femur/Tibia | Culture | 9.5% | 92.8% | |
B-cell (5) | Spleen | WGA (G-Plex) | 16.3% | 24.6% | |
NK-cell (1) | Spleen | WGA (G-Plex) | 11.1% | 13.4% | |
ML7 (67) | Satellite cell (57) | GAS (46): Left (23, 4 fibers) | Culture | 12.9% | 64.0% |
Age: 5.5w | Right (23, 5 fibers) | ||||
EDL (11): Left (3, 2 fibers) | |||||
Right (8, 3 fibers) | |||||
B-cell (10) | Spleen | WGA (G-Phi) | 13.6% | 69.3% |
Number of cells in each experiment from each cell type and organ source are indicated in brackets. Only cells which participated in lineage analysis are shown. % Amp. = percent of alleles which were successfully amplified on average from each cell type (based on manual analysis of signals); % Mut. = Percent of mutated alleles with respect to putative zygote (out of amplified alleles). w = weeks; SC = Stem Cell; EDL = Extensor Digitorum Longus muscle; SOL = Soleus muscle; GAS = Gastrocnemius muscle; MAS = Masseter muscle; WGA = Whole Genome Amplification; G-Plex = GenomePlex; G-Phi = GenomiPhi.