Fig. 4.
Delayed migration of FITC+ Langerhans cells (LC) into the draining lymph node (LN) of ethanol (EtOH)-fed mice. Abdominal skin was FITC painted and inguinal LN cells harvested for flow cytometry. (A) Gating strategy for identification of FITC+ LC in LN. (B–D) Comparison of FITC+ LC accumulation in LN of control or 4, 8 or 16 weeks EtOH-fed mice. n = 16 mice at 12 and 24 hours and n = 5 to 6 mice for remaining time points. *Indicates p(2) ≤ 0.05 for control versus EtOH at the specified time point. Error bars represent standard error of the mean.