Fig. 3.
Over-expression of Wnt-1 prevents β-amyloid (Aβ)-induced DNA fragmentation and membrane phosphatidylserine (PS) exposure in human neuroblastoma SH-SY5Y cells. Representative images of SH-SY5Y cells illustrate DNA fragmentation with terminal deoxynucleotidyl transferase nick end labeling (TUNEL) and phosphatidylserine (PS) exposure with annexin V phycoerythrin labeling in SH-SY5Y cells 24 h after administration of Aβ (20 μM). Aβ yielded significant DNA fragmentation and membrane PS exposure when compared to control untreated cultures. In contrast, overexpression of Wnt-1 results in a significant reduction in DNA fragmentation and PS exposure.