Skip to main content
. 2008 Jan 10;149(4):1728–1735. doi: 10.1210/en.2007-0826

Figure 1.

Figure 1

CITED1 expression in osteoblasts. A, Wt9 cells were treated with 100 nm hPTH(1–34) for 4 h before extraction of RNA and subsequent measurement of CITED1, CITED2, and CITED4 mRNA expression by quantitative RT-PCR, as described in Materials and Methods. Results are expressed as fold over control. B, Primary osteoblasts isolated from neonatal C57BL6 mice were treated with 100 nm hPTH(1–34) for 4 h before RNA extraction and measurement of CITED1 mRNA expression, as in A. C, Wt9 cells were treated with 30 nm hPTH(1–34), or vehicle alone (control), for 6 h or 24 h before simultaneous extraction of proteins in radioimmunoprecipitation assay buffer and assessment of CITED1 expression by Western blot analysis. D, Intensities of CITED1 and β-actin protein bands from five experiments performed as in C were assessed densitometrically (see Materials and Methods) and are presented as fold over the corresponding 6 h control in each experiment. Data are shown as mean ± sd for groups of three cultures (A and B). *, P < 0.01 vs. control (CON).