Figure 3.

PTH increases CITED1 expression in a dose- and time-dependent manner in osteoblasts. Wt9 cells were treated with 100 nm hPTH(1–34) for indicated times (A) or 4 h with hPTH(1–34) or G¹R¹⁹(1–28) at the indicated concentrations (B). Total RNA was extracted and the expression of CITED1 was measured by quantitative RT-PCR and expressed as fold over control. Experiments were performed three times with similar results. Data are shown as mean ± sd for groups of three cultures. *, P < 0.01 vs. control (CON).