Figure 6.

Role of CITED1 in regulation of osteoblastic differentiation by intermittent PTH in 3-wk cultures. Primary osteoblastic cells from WT or CITED1 KO mice were plated in type I collagen-coated 24-well plates and cultured until 90% confluent before initiation of cyclic (4 h every 48 h) PTH treatment (see Materials and Methods), using the peptides and concentrations indicated. After 3 wk, mineralization was assessed by Alizarin Red-S staining (top panel) and calcium content was measured in acid extracts of the cultures (bottom panel). Bars depict means ± se of three independent experiments. a, P < 0.01 vs. same-genotype control; b, P < 0.01 vs. G¹R¹⁹(1–28) in same genotype; c, P < 0.01 vs. WT control.