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. 2008 Jan 3;149(4):1820–1828. doi: 10.1210/en.2007-0993

Figure 6.

Figure 6

Molecular markers of growth plate senescence. Female rats were made hypothyroid by adding PTU to the drinking water from birth until 8 wk of age. Untreated animals served as controls. All rats received depot leuprolide acetate every 6 wk, starting at 3 wk of age. The solid boxes represent time animals received PTU (0–8 wk of age, pertains to all graphs). The relative concentration (mean ± sem) of each mRNA is presented relative to the average concentration found in 3-wk-old control animals and adjusted for the amount of starting cDNA (using the calibrator gene 18S rRNA) as well as the efficiency of the real-time PCR. The levels of chondroadherin, osteoprotegerin, secreted frizzled-related protein 4, reelin, and nuclear protein 1 mRNA increased with age in microdissected growth plates collected from control animals (*, P < 0.001, by ANOVA). At 11 wk of age, the age-dependent rise in mRNA expression was delayed in the animals that had previously received PTU (by t test, P values shown at right side of each panel).