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Impaired leptin signaling in ASKO mice. A, Glycerol released from WT and ASKO cells stimulated with varying doses of rLeptin or isoproterenol. Data are means ± se from triplicate dishes repeated three times. B, Western blot analysis of STAT1 tyrosine phosphorylation in sc primary preadipocytes isolated from WT and ASKO mice cultured in the presence of 10.0 ng/ml rLeptin for 24 h.
