Figure 5.

Western analysis for actin, tyrosinated tubulin, FasL, and Fas. A, Testicular proteins (100 μg) in lysates from male rats treated with a single oral dose of l-CDB-4022 (2.5 mg/kg) or vehicle (10% ethanol/sesame oil) at 45 d of age were resolved by SDS-PAGE, transferred to nitrocellulose membranes, and immunostained with actin, tyrosinated tubulin, FasL, and Fas antibodies. B, The immunoblots were densitometrically scanned for the protein levels of FasL (top panel) and Fas (bottom panel). The levels of FasL and Fas in control (Ctrl) rats were arbitrarily set at 1.0. Each bar is mean ± se of data from four blots (four rats per time point). *, P < 0.05.