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Inhibition of feeding by central leptin in AGRP+POMC:Lepr−/− mice. The cumulative food intake of male mice (control and AGRP+POMC:Lepr−/−) fitted with lateral ventricular cannulae was monitored for up to 23 h. Doses of leptin (2.5, 5, and 10 μg or vehicle) were injected immediately before monitoring intake. *, Statistically significant difference from the vehicle treatment. There were six mice in each group and the data are presented as means and sds.
