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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Methods. 2008 Feb;44(2):81–86. doi: 10.1016/j.ymeth.2007.09.003

Figure 1.

Figure 1

Acid gel electrophoresis resolves aminoacyl-tRNA from deacylated tRNA, allowing the extent of aminoacylation to be determined. (A) Various amounts (as indicated) of tRNAVal, Val-tRNAVal, and N-acetyl-Val-tRNAVal were subjected to acid gel electrophoresis, the gel was stained with methylene blue (top panel), dried, exposed to a phosphorimager screen, and scanned (bottom panel). (B) Various amounts of tRNAfMet and fMet-tRNAfMet were resolved on an acid gel, which was then stained with methylene blue.